t cells contribute to tumor progression by favoring pro-tumoral properties of intra-tumoral myeloid cells in a mouse model for spontaneous melanomat细胞促进肿瘤进展通过支持pro-tumoral属性的肿瘤内髓细胞自发的黑色素瘤的小鼠模型.pdf

T Cells Contribute to Tumor Progression by Favoring Pro- Tumoral Properties of Intra-Tumoral Myeloid Cells in a Mouse Model for Spontaneous Melanoma ´ 1,2,3. 1,2,3. 1,2,3 1,2,3 ` Renee Lengagne , Arnaud Pommier , Jonathan Caron , Laetitia Douguet , Marylene 1,2,3 4 1,2,3,5 6 ` 1,2,3 Garcette , Masashi Kato , Marie-Franc¸oise Avril , Jean-Pierre Abastado , Nadege Bercovici , 1,2,3 ´ 1,2,3 Bruno Lucas , Armelle Prevost-Blondel * 1 INSERM, U1016, Institut Cochin, Paris, France, 2 CNRS, UMR8104, Paris, France, 3 University Paris Descartes, Paris, France, 4 Unit of Environmental Health Sciences, Chubu ˆ University, Aichi, Japan, 5 APHP, Hopital Cochin, Service de Dermatologie, Paris, France, 6 Singapore Immunology Network, BMSI, A-STAR, Singapore, Singapore Abstract Tumors affect myelopoeisis and induce the expansion of myeloid cells with immunosuppressive activity. In the MT/ret model of spontaneous metastatic melanoma, myeloid cells are the most abundant tumor infiltrating hematopoietic population and their proportion is highest in the most aggressive cutaneous metastasis. Our data suggest that the tumor microenvironment favors polarization of myeloid cells into type 2 cells characterized by F4/80 expression, a weak capacity to secrete IL-12 and a high production of arginase. Myeloid cells from tumor and spleen of MT/ret mice inhibit