yersinia enterocolitica targets cells of the innate and adaptive immune system by injection of yops in a mouse infection model鼠疫enterocolitica目标细胞的先天和适应性免疫系统注入yops小鼠感染模型.pdf

Yersinia enterocolitica Targets Cells of the Innate and Adaptive Immune System by Injection of Yops in a Mouse Infection Model ¨ 1 ¨ 1 ¨ 1 1 1 Martin Koberle , Annegret Klein-Gunther , Monika Schutz , Michaela Fritz , Susanne Berchtold , Eva 2,3 1 1 Tolosa , Ingo B. Autenrieth , Erwin Bohn * ¨ ¨ ¨ ¨ ¨ 1 Institut fur Medizinische Mikrobiologie und Hygiene, Universitatsklinikum Tubingen, Tubingen, Germany, 2 Hertie-Institut fur klinische Hirnforschung, ¨ ¨ ¨ ¨ ¨ Universitatsklinikum Tubingen, Tubingen, Germany, 3 Zentrum fur Molekulare Neurobiologie, Universitat Hamburg, Hamburg, Germany Abstract Yersinia enterocolitica (Ye) evades the immune system of the host by injection of Yersinia outer proteins (Yops) via a type three secretion system into host cells. In this study, a reporter system comprising a YopE-b-lactamase hybrid protein and a fluorescent staining sensitive to b-lactamase cleavage was used to track Yop injection in cell culture and in an experimental Ye mouse infection model. Experiments with GD25, GD25-b1A, and HeLa cells demonstrated that b1-integrins and RhoGTPases play a role for Yop injection. As demonstrated by infection of splenocyte suspensions in vitro, injection of Yops appears to occur randomly into all types of leukocytes. In contrast, upon infection of mice, Yop injection was detected in + + + +