the armadillo repeat protein pf16 is essential for flagellar structure and function in plasmodium male gametes犰狳重复蛋白pf16是必不可少的疟原虫雄性配子的鞭毛结构和功能.pdf

The Armadillo Repeat Protein PF16 Is Essential for Flagellar Structure and Function in Plasmodium Male Gametes 1,2. 2. 3 1 1 Ursula Straschil , Arthur M. Talman , David J. P. Ferguson , Karen A. Bunting , Zhengyao Xu , 1 2 4 5 6 Elizabeth Bailes , Robert E. Sinden , Anthony A. Holder , Elizabeth F. Smith , Juliet C. Coates , Rita Tewari1,2* 1 Institute of Genetics, School of Biology, University of Nottingham, Nottingham, United Kingdom, 2 Division of Cell and Molecular Biology, Imperial College London, London, United Kingdom, 3 Nuffield Department of Clinical Laboratory Science, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom, 4 Division of Parasitology, MRC National Institute for Medical Research, London, United Kingdom, 5 Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, United States of America, 6 School of Biosciences, University of Birmingham, Birmingham, United Kingdom Abstract Malaria, caused by the apicomplexan parasite Plasmodium, threatens 40% of the world’s population. Transmission between vertebrate and insect hosts depends on the sexual stages of the life-cycle. The male gamete of Plasmodium parasite is the only developmental stage that possesses a flagellum. Very little is known about the identity or function of proteins in the parasite’s flagellar biology. Here, we characterise a Plasmodium PF16 homologue using reverse genetics in the mouse malaria parasite Plasmodium berghei. PF16 is a conserved Armadillo-repeat protein that regulates flagellar str