the glutathione biosynthetic pathway of plasmodium is essential for mosquito transmission谷胱甘肽的生物合成途径疟原虫的蚊子传播至关重要.pdf

The Glutathione Biosynthetic Pathway of Plasmodium Is Essential for Mosquito Transmission ´ 1 2. 3. 2 Joel Vega-Rodrıguez , Blandine Franke-Fayard , Rhoel R. Dinglasan , Chris J. Janse , Rebecca 1 2,4 3 ´ ´ 5 Pastrana-Mena , Andrew P. Waters , Isabelle Coppens , Jose F. Rodrıguez-Orengo , Marcelo Jacobs- 3 1 Lorena , Adelfa E. Serrano * 1 Department of Microbiology and Medical Zoology, University of Puerto Rico, School of Medicine, San Juan, Puerto Rico, 2 Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands, 3 Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, United States of America, 4 Wellcome Trust Centre of Molecular Parasitology and Division of Infection and Immunity, University of Glasgow, Glasgow, United Kingdom, 5 Department of Biochemistry, University of Puerto Rico, School of Medicine, San Juan, Puerto Rico Abstract Infection of red blood cells (RBC) subjects the malaria parasite to oxidative stress. Therefore, efficient antioxidant and redox systems are required to prevent damage by reactive oxygen species. Plasmodium spp. have thioredoxin and glutathione (GSH) systems that are thought to play a major role as antioxidants during blood stage infection. In this report, we analyzed a critical component of the GSH biosynthesis pathway using reverse genetics. Plasmodium berghei parasites lacking expression of gamma-glutamylcysteine synthetase (c-GCS), the rate limiting e