comparative evolution of s7 intron 1 and ribosomal internal transcribed spacer in coilia nasus (clupeiformes engraulidae)比较s7的进化基因内区1和核糖体内部转录间隔区coilia nasus(鲱形目鳀科).pdf

Int. J. Mol. Sci. 2012, 13, 3085-3100; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Comparative Evolution of S7 Intron 1 and Ribosomal Internal Transcribed Spacer in Coilia nasus (Clupeiformes: Engraulidae) Dong Liu, Hong-Yi Guo, Wen-Qiao Tang * and Jin-Quan Yang College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; E-Mails: dliu@ (D.L.); hy-guo@ (H.-Y.G.); jqyang@ (J.-Q.Y.) * Author to whom correspondence should be addressed; E-Mail: wqtang@; Tel./Fax: +86-21-6190-0425. Received: 21 October 2011; in revised form: 17 January 2012 / Accepted: 28 February 2012 / Published: 7 March 2012 Abstract: Coilia nasus is widely distributed in the Yangtze River, the coastal waters of China, Korea and the Ariake Sound of Japan. Several ecotypes exist and this provides a useful model for the study of comparative diversity between molecular markers. Here we analyze and compare the nucleotide sequences between single-copy ribosomal protein S7 gene intron 1 (rpS7) and multiple-copy ribosomal internal transcribed spacer 1 (ITS1) in this species to compare the phylogenetic signal of the two nuclear genes. Nucleotide substitutions among the two gene sequences and partial sequence of mitochondrial cytochrome c oxidase subunit I (COI) gene were also analyzed. A total of 115