comparative analysis of flc homologues in brassicaceae provides insight into their role in the evolution of oilseed rape比较分析的方法同系物在十字花科提供洞察他们的角色在油菜的进化.pdf

Comparative Analysis of FLC Homologues in Brassicaceae Provides Insight into Their Role in the Evolution of Oilseed Rape 1 2 3 1 1 1 2 Xiaoxiao Zou , Ida Suppanz , Harsh Raman , Jinna Hou , Jing Wang , Yan Long , Christian Jung , Jinling Meng1* 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China, 2 Plant Breeding Institute, Christian-Albrechts-University of Kiel, Kiel, Germany, 3 EH Graham Centre for Agricultural Innovation (an alliance between the Charles Sturt University and New South Wales Department of Primary Industries), Wagga Wagga Agricultural Institute, Wagga Wagga, New South Wales, Australia Abstract We identified nine FLOWERING LOCUS C homologues (BnFLC) in Brassica napus and found that the coding sequences of all BnFLCs were relatively conserved but the intronic and promoter regions were more divergent. The BnFLC homologues were mapped to six of 19 chromosomes. All of the BnFLC homologues were located in the collinear region of FLC in the Arabidopsis genome except BnFLC.A3b and BnFLC.C3b, which were mapped to noncollinear regions of chromosome A3 and C3, respectively. Four of the homologues were associated significantly with quantitative trait loci for flowering time in two mapping populations. The BnFLC homologues showed distinct expression patterns in vegetative and reproductive organs, and at different developmental stages. BnFLC.A3b was differentially expressed between the winter-type and semi-winter- type cultivars. Microsynteny analysis indicated that BnFLC.A3b might have been translocated to the present segment in a cluster with other flowering-time regulators, such as a homologue of FRIGIDA in Arabidopsis. This cluster of flowering-time genes might hav