the gene expression profile in the synovium as a predictor of the clinical response to infliximab treatment in rheumatoid arthritis滑膜中的基因表达谱的预测英夫利昔单抗治疗类风湿性关节炎的临床反应.pdf

The Gene Expression Profile in the Synovium as a Predictor of the Clinical Response to Infliximab Treatment in Rheumatoid Arthritis 1 2 2 3 3 Johan Lindberg , Carla A. Wijbrandts , Lisa G. van Baarsen , Gustavo Nader , Lars Klareskog , Anca 3 2 2 1 2 Catrina , Rogier Thurlings , Margriet Vervoordeldonk , Joakim Lundeberg , Paul P. Tak * 1 Department of Gene Technology, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden, 2 Division of Clinical Immunology and Rheumatology, Academic Medical Center/University of Amsterdam, Amsterdam, The Netherlands, 3 Rheumatology Unit, Department of Medicine, Karolinska Institute, Karolinska University Hospital, Solna, Sweden Abstract Background: Although the use of TNF inhibitors has fundamentally changed the way rheumatoid arthritis (RA) is treated, not all patients respond well. It is desirable to facilitate the identification of responding and non-responding patients prior to treatment, not only to avoid unnecessary treatment but also for financial reasons. In this work we have investigated the transcriptional profile of synovial tissue sampled from RA patients before anti-TNF treatment with the aim to identify biomarkers predictive of response. Methodology/Principal Findings: Synovial tissue samples were obtained by arthroscopy from 62 RA patients before the initiation of infliximab treatment. RNA was extracted and gene expression profiling was performed using an in-house spotted long oligonucleotide array covering 17972 unique genes. Tissue sections were also anal