swelling-activated anion channels are essential for volume regulation of mouse thymocytesswelling-activated阴离子通道音量调节的小鼠胸腺细胞至关重要.pdf

Int. J. Mol. Sci. 2011, 12, 9125-9137; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Swelling-Activated Anion Channels Are Essential for Volume Regulation of Mouse Thymocytes Ranokhon S. Kurbannazarova 1,2,3, Svetlana V. Bessonova 1,2, Yasunobu Okada 1,* and Ravshan Z. Sabirov 1,2,3 1 Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan; E-Mails: psharapovna@ (R.S.K.); bessosvetlana@mail.ru (S.V.B.); sabirov@nips.ac.jp (R.Z.S.) 2 Laboratory of Molecular Physiology, Institute of Physiology and Biophysics, Acad. Sci. RUz, Tashkent 100095, Niyazova 1, Uzbekistan 3 Department of Biophysics, National University, Tashkent 100174, Vuzgorodok, Uzbekistan * Author to whom correspondence should be addressed; E-Mail: okada@nips.ac.jp; Tel.: +81-564-55-7731; Fax: +81-564-55-7735. Received: 25 October 2011; in revised form: 10 November 2011 / Accepted: 24 November 2011 / Published: 8 December 2011 Abstract: Channel-mediated trans-membrane chloride movement is a key process in the active cell volume regulation under osmotic stress in most cells. However, thymocytes were hypothesized to regulate their volume by activating a coupled K-Cl cotransport mechanism. Under the patch-clamp, we found that osmotic swelling activates two types of macroscopic anion conductance with different voltage-