tbunc119 and its binding protein complex are essential for propagation, motility, and morphogenesis of trypanosoma brucei procyclic form cellstbunc119及其结合蛋白复杂传播至关重要,能动性,形态发生锥体虫属brucei亦形成细胞.pdf

TbUNC119 and Its Binding Protein Complex Are Essential for Propagation, Motility, and Morphogenesis of Trypanosoma brucei Procyclic Form Cells 1,2,3. 2,3,4. 5 3 3 Shigeru Ohshima , Mitsuko Ohashi-Suzuki , Yutaka Miura , Yoshisada Yabu , Noriko Okada , Nobuo Ohta2,4, Takashi Suzuki2,3,4* 1 Department of Core Laboratory, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan, 2 Department of Molecular Parasitology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan, 3 Department of Immunology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan, 4 Division of Public Health, Department of International Health Development, Graduate School of Tokyo Medical and Dental University, Tokyo, Japan, 5 Department of Molecular Neurology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan Abstract Flagellum-mediated motility of Trypanosoma brucei is considered to be essential for the parasite to complete stage development in the tsetse fly vector, while the mechanism by which flagellum-mediated motility is controlled are not fully understood. We thus compared T. brucei whole gene products (amino acid sequence) with Caenorhabditis elegans UNC (uncoordinated) proteins, in order to find uncharacterized motility-related T. brucei genes. Through in silico analysis, we found 88 gene products which were highly similar to C. elegans UNC proteins and categorized them as TbCEUN (T. brucei gene products which have high similarity to C. elegans UNC proteins). Approximately two thirds of the 88 TbCEUN gene products were kinesin-related molecules. A gene