at-risk and recent-onset type 1 diabetic subjects have increased apoptosis in the cd4+cd25+high t-cell fraction危险和最近研究1型糖尿病患者增加了细胞凋亡的cd4 + cd25 + t细胞比例高.pdf

At-Risk and Recent-Onset Type 1 Diabetic Subjects Have Increased Apoptosis in the CD4+CD25+high T-Cell Fraction 1 1 1 1 1 2 3 1 Sanja Glisic-Milosavljevic , Jill Waukau , Parthav Jailwala , Srikanta Jana , Huoy-Jii Khoo , Hope Albertz , Jeffrey Woodliff , Marilyn Koppen , 4 5 1 Ramin Alemzadeh , William Hagopian , Soumitra Ghosh * 1The Max McGee National Center for Juvenile Diabetes and Human Molecular Genetics Center, Medical College of Wisconsin and the Children’s Research Institute of the Children’s Hospital of Wisconsin, Milwaukee, Wisconsin, United States of America, 2 Blood Center of Wisconsin, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America, 3 Flow Cytometry Core Facility, Department of Pediatrics, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America, 4 Children’s Hospital of Wisconsin Diabetes Center, Pediatric Endocrinology and Metabolism, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America, 5 Pacific Northwest Research Institute, Seattle, Washington, United States of America Background. In experimental models, Type 1 diabetes (T1D) can be prevented by adoptive transfer of CD4+CD25+ (FoxP3+) suppressor or regulatory T cells. Recent studies have found a suppression defect of CD4+CD25+high T cells in human disease. In this study we measure apoptosis of CD4+CD25+high T cells to see if it could contribute to reduced suppressive activity of these cells. Methods and Findings. T-cell apoptosis was evaluated in children and adolescent (35 females/40 males) subjects comprising recent-onset and long-standing T1D subjects and their first-degree relatives, who are at variable risk to develop