zeb2 mediates multiple pathways regulating cell proliferation, migration, invasion, and apoptosis in gliomazeb2介导多种通路调节细胞增殖、迁移,侵略,并在神经胶质瘤细胞凋亡.pdf

ZEB2 Mediates Multiple Pathways Regulating Cell Proliferation, Migration, Invasion, and Apoptosis in Glioma 1 . 1,2. 1. 2. 1 2 1 Songtao Qi * , Ye Song , Yuping Peng , Hao Wang , Hao Long , Xiaoli Yu , Zhiyong Li , 1 2 2 2 2 2 2 Luxiong Fang , Aibing Wu , Weiren Luo , Yan Zhen , Ying Zhou , Yan Chen , Chunping Mai , 2,3 2 Zhen Liu *, Weiyi Fang * 1 Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China, 2 Cancer Research Institute of Southern Medical University, Guangzhou, People’s Republic of China, 3 Department of Pathology, Basic School of Guangzhou Medical College, Guangzhou, People’s Republic of China Abstract Background: The aim of the present study was to analyze the expression of Zinc finger E-box Binding homeobox 2 (ZEB2) in glioma and to explore the molecular mechanisms of ZEB2 that regulate cell proliferation, migration, invasion, and apoptosis. Methodology/Principal Findings: Expression of ZEB2 in 90 clinicopathologically characterized glioma patients was analyzed by immunohistochemistry. Furthermore, siRNA targeting ZEB2 was transfected into U251 and U87 glioma cell lines in vitro and proliferation, migration, invasion, and apoptosis were examined separately by MTT assay, Transwell chamber assay, flow cytometry, and western blot. Results: The expression level of ZEB2 protein was significantly increased in glioma tissues compared to normal brain tissues (P,0.001). In addition, high levels of ZEB2 protein were positively correlated with pathology grade classification (P = 0.024) of gli