the combination of trail and isoflavones enhances apoptosis in cancer cells小路和异黄酮增强癌细胞凋亡.pdf

Molecules 2010, 15, 2000-2015; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells † † Joanna Bronikowska , Ewelina Szliszka , Zenon P. Czuba, Dariusz Zwolinski, Dariusz Szmydki and Wojciech Krol * Chair and Department of Microbiology and Immunology, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland; E-Mails: jbronikowska@.pl (J.B.); eszliszka@.pl (E.S.); zczuba@.pl (Z.P.C.); 6132@wp.pl (D.Z.); dariusz.szmydki@op.pl (D.S.) † These authors contributed equally to this work. * Author to whom correspondence should be addressed; E-Mail: wkrol@.pl; Tel./Fax: + 480322722554. Received: 4 February 2010; in revised form: 9 March 2010 / Accepted: 19 March 2010 / Published: 22 March 2010 Abstract: Isoflavones are a class of bioactive polyphenols with cancer chemopreventive properties. TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) is a naturally occurring antitumor agent that selectively induces programmed death (apoptosis) in cancer cells. Polyphenols can modulate TRAIL-mediated apoptosis in cancer cells. We examined the cytotoxic and apoptotic activities of isoflavones in combination with TRAIL on HeLa cancer cells. The apoptosis was detected by fluorescence microscopy with annexin V-FITC. The cytotoxicity was evaluated by MTT and LDH assays. The tested isoflavones: genistein, biochanin-A and neobavaisoflavone enhance TRAI