树鼩Tssk6基因cDNA克隆、组织表达谱+及生物信息学分析.pdf

2017 44 3 619627 China A nim al H usbandry Vete r inary M e d ic ine doi cnki j Tss k6 cD N A Tssk cDNA GenBank Tssk mRNA Tssk cDNA PCR cDNA CDS Tssk cDNA CDS bp Tssk Tssk cDNA CDS Tssk Tssk cDNA PCR R A cDNA Cloning Tissue Expression Profiles and Bioinformatical Analysis of Tssk6 Gene in Tree Shrews LI Yuani YANG Minghua LIU Fang Z HU Fangfang j HE Shao u KUANG Haiou LI Yahui y Faculty of A nimal Science and Technology Yunnan A g ricultural Univ ersity K unming China College of Food Science and Technology Yunnan A gricultural Univ ersity K unming China Abstract T he purpo se of this study w as to clone the f ull leng th cDN A of tree shrew s Tssk gene to elucidate it s tissue ex pression profiles and f undamental bioinf ormatical characteristics In the present study the f ullleng th cDN A of tree shrew s Tssk ene w as cloned w ith specific g rimers on the basis of predicted m RN A sequence of tree shrew s Tssk ene in GenBank the p g m RN A ex pression of Tssk ene in various tree shrew s tissues w as investigated v ia Realtime g PCR In addition the bioinf ormatics on nucleotide sequence of CDS region of T ssk cDNA in