components of the hematopoietic compartments in tumor stroma and tumor-bearing mice组件造血隔间在肿瘤间质肿瘤的老鼠.pdf

Components of the Hematopoietic Compartments in Tumor Stroma and Tumor-Bearing Mice 1. 1. 1 1 2 HoangDinh Huynh , Junke Zheng , Masato Umikawa , Robert Silvany , Xian-Jin Xie , Catherine J. 3 4 5 1 Wu , Martin Holzenberger , Qianming Wang , Cheng Cheng Zhang * 1 Departments of Physiology and Developmental Biology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, 2 Department of Clinical Sciences, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, 3 Dana-Farber Cancer Institute, Boston, Massachusetts, United States of America, 4 INSERM U515, Hopital St-Antoine, Paris, France, 5 School of Chemistry and Environment, South China Normal University, Guangzhou, People’s Republic of China Abstract Solid tumors are composed of cancerous cells and non-cancerous stroma. A better understanding of the tumor stroma could lead to new therapeutic applications. However, the exact compositions and functions of the tumor stroma are still largely unknown. Here, using a Lewis lung carcinoma implantation mouse model, we examined the hematopoietic compartments in tumor stroma and tumor-bearing mice. Different lineages of differentiated hematopoietic cells existed in tumor stroma with the percentage of myeloid cells increasing and the percentage of lymphoid and erythroid cells decreasing over time. Using bone marrow reconstitution analysis, we showed that the tumor stroma also contained functional hematopoietic stem cells. All hematopoietic cells in the tumor stroma