complete genome sequence of mycoplasma suis and insights into its biology and adaption to an erythrocyte niche支原体的完整基因组序列是,洞察其生物学和适应红细胞利基.pdf

Complete Genome Sequence of Mycoplasma suis and Insights into Its Biology and Adaption to an Erythrocyte Niche 1,2 1 3 4 5 Ana M. S. Guimaraes *, Andrea P. Santos , Phillip SanMiguel , Thomas Walter , Jorge Timenetsky , Joanne B. Messick1* 1 Department of Comparative Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana, United States of America, 2 CAPES-Fulbright ´ ˜ ´ Program, Ministerio da Educac¸ao, Brasılia, Brazil, 3 Purdue Genomics Core Facility, Purdue University, West Lafayette, Indiana, United States of America, 4 Department of Biological Sciences, Purdue University, West Lafayette, Indiana, United States of America, 5 Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Brazil Abstract Mycoplasma suis, the causative agent of porcine infectious anemia, has never been cultured in vitro and mechanisms by which it causes disease are poorly understood. Thus, the objective herein was to use whole genome sequencing and analysis of M. suis to define pathogenicity mechanisms and biochemical pathways. M. suis was harvested from the blood of an experimentally infected pig. Following DNA extraction and construction of a paired end library, whole-genome sequencing was performed using GS-FLX (454) and Titanium chemistry. Reads on paired-end constructs were assembled using GS De Novo Assembler and gaps closed by primer walking; assembly was validated by PFGE. Glimmer and Manatee Annotation Engine were used to predict and annotate protein-coding sequences (CDS). The