complete chloroplast genome sequence of an orchid model plant candidate erycina pusilla apply in tropical oncidium breeding兰花模型的完整的叶绿体基因组序列植物候选人erycina pusilla适用于热带金蝶兰属植物育种.pdf

Complete Chloroplast Genome Sequence of an Orchid Model Plant Candidate: Erycina pusilla Apply in Tropical Oncidium Breeding 1,2,3 2 2 4 4 I-Chun Pan , Der-Chih Liao , Fu-Huei Wu , Henry Daniell , Nameirakpam Dolendro Singh , 5 2 1,2,3 2 Chen Chang , Ming-Che Shih , Ming-Tsair Chan *, Choun-Sea Lin * 1 Institute of Biotechnology, National Cheng Kung University, Tainan, Taiwan, 2 Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan, 3 Academia Sinica Biotechnology Center in Southern Taiwan, Tainan, Taiwan, 4 Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, Florida, United States of America, 5 Department of Horticulture, National Chung Hsing University, Taichung, Taiwan Abstract Oncidium is an important ornamental plant but the study of its functional genomics is difficult. Erycina pusilla is a fast- growing Oncidiinae species. Several characteristics including low chromosome number, small genome size, short growth period, and its ability to complete its life cycle in vitro make E. pusilla a good model candidate and parent for hybridization for orchids. Although genetic information remains limited, systematic molecular analysis of its chloroplast genome might provide useful genetic information. By combining bacterial artificial chromosome (BAC) clones and next-generation sequencing (NGS), the chloroplast (cp) genome of E. pusilla was sequenced accurately, efficiently and economically. The cp genome of E. pusilla shares 89 and 84% similarity with Oncidium Gower Ramsey and Phalanopsis aphrodite, respectively.