structural analysis of cytochrome p450 105n1 involved in the biosynthesis of the zincophore, coelibactin结构分析的细胞色素p450 105 n1 zincophore参与生物合成,coelibactin.pdf

Int. J. Mol. Sci. 2012, 13, 8500-8513; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Structural Analysis of Cytochrome P450 105N1 Involved in the Biosynthesis of the Zincophore, Coelibactin Bin Zhao 1,*, Suzy C. Moody 2, Robert C. Hider 3, Li Lei 1, Steven L. Kelly 2, Michael R. Waterman 1 and David C. Lamb 2,* 1 Department of Biochemistry, School of Medicine, Vanderbilt University, Nashville, TN 37232, USA; E-Mails: li.lei@ (L.L.); michael.waterman@ (M.R.W.) 2 Institute of Life Science, Medical School, Swansea University, Singleton Park, Swansea, SA2 8PP, UK; E-Mails: s.moody.562842@swansea.ac.uk (S.C.M.); s.l.kelly@swansea.ac.uk (S.L.K.) 3 Institute of Pharmaceutical Science, King’s College London, 5th Floor, Franklin-Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK; E-Mail: robert.hider@kcl.ac.uk * Authors to whom correspondence should be addressed; E-Mails: bin.zhao@ (B.Z.); d.c.lamb@swansea.ac.uk (D.C.L.); Tel.: +1-615-322-2414 (B.Z.); +44-1792-602178 (D.C.L.); Fax: +1-615-343-0704 (B.Z.); +44-1792-602280 (D.C.L.). Received: 1 June 2012; in revised form: 22 June 2012 / Accepted: 28 June 2012 / Published: 9 July 2012 Abstract: Coelibactin is a putative non-ribosomally synthesized peptide with predicted zincophore activity and which has been implicated in antibiotic regulation in Streptomyces coelicolor A3(2). The coelibactin biosynthetic pathway contain