the characterisation of aop2 a gene associated with the biosynthesis of aliphatic alkenyl glucosinolates in arabidopsis thalianaaop2的描述与脂肪族烯基硫配糖体的生物合成相关的基因在拟南芥.pdf

Neal et al. BMC Plant Biology 2010, 10:170 /1471-2229/10/170 RESEARCH ARTICLE Open Access The characterisation of AOP2: a gene associated with the biosynthesis of aliphatic alkenyl glucosinolates in Arabidopsis thaliana 1,2 1,3 1 1* Calida S Neal , Dale P Fredericks , Cara A Griffiths , Alan D Neale Abstract Background: Glucosinolates, a group of nitrogen and sulfur containing compounds associated with plant-insect interactions, are produced by a number of important Brassicaceae crop species. In Arabidopsis the AOP2 gene plays a role in the secondary modification of aliphatic (methionine-derived) glucosinolates, namely the conversion of methylsulfinylalkyl glucosinolates to form alkenyl glucosinolates, and also influences aliphatic glucosinolate accumulation. Results: This study characterises the primary structural variation in the coding sequences of the AOP2 gene and identifies three different AOP2 alleles based on polymorphisms in exon two. To help determine the regulatory mechanisms mediating AOP2 expression amongst accessions, AOP2 5′ regulatory regions were also examined however no major differences were identified. Expression of the AOP2 gene was found to be most abundant in leaf and stem tissue and was also found to be light dependent, with a number of light regulatory elements identified in the promoter region of the gene. In addition, a study was undertaken to demonstrate that the Arabidopsis AOP2 gene product is functional in planta. The over-expression of a functional AOP2 allele was found to successfully convert the precursor methylsulfinyl alkyl glucosinolate into the alkenyl form. Conclusions: The expression of the AOP2 gene has been found to be influenced by light and is most highly expressed in the phot