comparative functional genomics of salt stress in related model and cultivated plants identifies and overcomes limitations to translational genomics比较功能基因组学相关的模型和栽培植物盐胁迫的转化基因识别和克服的局限性.pdf

Comparative Functional Genomics of Salt Stress in Related Model and Cultivated Plants Identifies and Overcomes Limitations to Translational Genomics 1¤a 2 1 1 Diego H. Sanchez , Fernando L. Pieckenstain , Jedrzey Szymanski , Alexander Erban , Mariusz 1 1¤b 3 1 4 Bromke , Matthew A. Hannah , Ute Kraemer , Joachim Kopka , Michael K. Udvardi * ´ ´ ´ 1 Max Planck Institute for Molecular Plant Physiology (MPIMP), Potsdam-Golm, Germany, 2 Instituto Tecnologico de Chascomus (IIB-Intech), Chascomus, Argentina, 3 Department of Plant Physiology, Ruhr University Bochum, Bochum, Germany, 4 Samuel Roberts Noble Foundation, Ardmore, Oklahoma, United States of America Abstract One of the objectives of plant translational genomics is to use knowledge and genes discovered in model species to improve crops. However, the value of translational genomics to plant breeding, especially for complex traits like abiotic stress tolerance, remains uncertain. Using comparative genomics (ionomics, transcriptomics and metabolomics) we analyzed the responses to salinity of three model and three cultivated species of the legume genus Lotus. At physiological and ionomic levels, models responded to salinity in a similar way to crop species, and changes in the concentration of shoot Cl2 correlated well with tolerance. Metabolic changes were partially conserved, but divergence was observed amongst the genotypes. Transcriptome anal