comparative genomics and drug resistance of a geographic variant of st239 methicillin-resistant staphylococcus aureus emerged in russia比较基因组学和耐药性的地理变异st239耐甲氧西林金黄色葡萄球菌出现在俄罗斯.pdf

Comparative Genomics and Drug Resistance of a Geographic Variant of ST239 Methicillin-Resistant Staphylococcus aureus Emerged in Russia 1 1 1 1 1,2 1 Tatsuo Yamamoto *, Tomomi Takano , Wataru Higuchi , Yasuhisa Iwao , Olga Singur , Ivan Reva , 3 3 3 2 2 2 Yuta Otsuka , Toru Nakayashiki , Hirotada Mori , Galina Reva , Vladimir Kuznetsov , Vladimir Potapov 1 Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan, 2 Vladivostok State Medical University, Vladivostok, Russia, 3 Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan Abstract Two distinct classes of methicillin-resistant Staphylococcus aureus (MRSA) are spreading in hospitals (as hospital-acquired MRSA, HA-MRSA) and in the community (as community-acquired MRSA, CA-MRSA). Multilocus sequence type (ST) 239 MRSA, one of the most worldwide-disseminated lineages, has been noted as a representative HA-MRSA. Here, we isolated ST239 MRSA (spa type 3 [t037] and staphylococcal cassette chromosome mec [SCCmec] type III.1.1.1) and its novel variant with ST239/spa351 (t030)/SCCmecIII.1.1.4 (SCCmecIIIR) not only from hospitals but also from patients with urethritis in the community in Russia. The Russian variant (strain 16K) possessed a hybrid genome consisting of CC8 and CC30, similar to the ST239/spa3/SCCmecIII.1.1.1 HA-MRSA (TW20) genome, but with marked diversity. The 16K9 CC30 section had SCCmecIIIR car