comparative genotypic and phenotypic characterisation of methicillin-resistant staphylococcus aureus st398 isolated from animals and humans比较确定基因型和表型的耐甲氧西林金黄色葡萄球菌st398孤立的从动物和人类.pdf

Comparative Genotypic and Phenotypic Characterisation of Methicillin-Resistant Staphylococcus aureus ST398 Isolated from Animals and Humans 1,2 2 3,4 1 Dorota M. Jamrozy *, Mark D. Fielder , Patrick Butaye , Nick G. Coldham 1 Department of Bacteriology, Animal Health and Veterinary Laboratories Agency, New Haw, United Kingdom, 2 School of Life Sciences, Faculty of Science, Engineering and Computing, Kingston University, London, United Kingdom, 3 Unit of General Bacteriology, Veterinary and Agrochemical Research Centre, Brussels, Belgium, 4 Department of Pathology, Bacteriology and Poultry Diseases, Ghent University, Ghent, Belgium Abstract The high prevalence of methicillin-resistant Staphylococcus aureus (MRSA) ST398 among pigs in certain European countries and North America and its occurrence in other animal species raises a question concerning the molecular mechanisms mediating the success of this lineage. In this study a panel of S. aureus strains belonging to sequence type (ST) 5 (n = 4), ST8 (n = 5), ST15 (n = 5), ST22 (n = 8), clonal complex (CC) 30 (n = 8), CC97 (n = 8), CC130 (n = 4), CC151 (n = 4) and ST398 (n = 18) were screened by DNA microarray and PCR for the carriage of virulence and antimicrobial resistance genes. Isolates belonging to the same sequence type/clonal complex (ST/CC) were found to share similar virulence gene profiles. The ST398 lineage displayed the lowest content of virulence genes, which consisted mainly of genes detected among the majority or all of the analysed lineages. All MRSA ST398 isolates lacked accessory virulence genes that were detected in other ST/CC. In contrast to virulence genotype, the antimicrobial resistance