contribution of panton-valentine leukocidin in community-associated methicillin-resistant staphylococcus aureus pathogenesis贡献的金黄色葡萄球菌杀白细胞毒素community-associated耐甲氧西林金黄色葡萄球菌发病机理.pdf

Contribution of Panton-Valentine Leukocidin in Community-Associated Methicillin-Resistant Staphylococcus aureus Pathogenesis 1 2 1 1 2 Binh An Diep , Amy M. Palazzolo-Ballance , Pierre Tattevin , Li Basuino , Kevin R. Braughton , Adeline R. 2 3 3 2 2 1 Whitney , Liang Chen , Barry N. Kreiswirth , Michael Otto , Frank R. DeLeo , Henry F. Chambers * 1 Division of Infectious Diseases, Department of Medicine, San Francisco General Hospital, University of California San Francisco, San Francisco, California, United States of America, 2 Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America, 3 Public Health Research Institute and the University of Medicine and Dentistry of New Jersey, Newark, New Jersey, United States of America Abstract Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strains typically carry genes encoding Panton- Valentine leukocidin (PVL). We used wild-type parental and isogenic PVL-deletion (Dpvl) strains of USA300 (LAC and SF8300) and USA400 (MW2) to test whether PVL alters global gene regulatory networks and contributes to pathogenesis of bacteremia, a hallmark feature of invasive staphylococcal disease. Microarray and proteomic analyses revealed that PVL does not alter gene or protein expression, thereby demonstrating that any contribution of PVL to CA-MRSA pathogenesis is not mediated through interference of global gene regulatory networks. Inasmuch as a direct role for PVL in CA-M