tentative mapping of transcription-induced interchromosomal interaction using chimeric est and mrna data初步的映射transcription-induced染色体间交互使用嵌合est和mrna数据.pdf

Tentative Mapping of Transcription-Induced Interchromosomal Interaction using Chimeric EST and mRNA Data Per Unneberg*, Jean-Michel Claverie Structural and Genomic Information Laboratory, Centre National de la Recherche Scientifique (CNRS) UPR-2589, Institut de Biologie Structurale et Microbiologie, Marseille, France Recent studies on chromosome conformation show that chromosomes colocalize in the nucleus, bringing together active genes in transcription factories. This spatial proximity of actively transcribing genes could provide a means for RNA interaction at the transcript level. We have screened public databases for chimeric EST and mRNA sequences with the intent of mapping transcription-induced interchromosomal interactions. We suggest that chimeric transcripts may be the result of close encounters of active genes, either as functional products or ‘‘noise’’ in the transcription process, and that they could be used as probes for chromosome interactions. We have found a total of 5,614 chimeric ESTs and 587 chimeric mRNAs that meet our selection criteria. Due to their higher quality, the mRNA findings are of particular interest and we hope that they may serve as food for thought for specialists in diverse areas of molecular biology. Citation: Unneberg P, Claverie J-M (2007) Tentative Mapping of Transcription-Induced Interchromosomal Interaction using Chimeric EST and mRNA Data. PLoS ONE 2(2): e254. doi:10.1371/journal.pone.0000254 INTRODUCTION fact, the chromosome is now known to be a highly mobile With the development of increasingly sophisticated large-scale structure, with territories intermingling significantly in the nucleus sequencing and microarray techniques, the known transcriptome [16]. continues to grow. Even though the idea that one gene produces