complex evolutionary history of the aeromonas veronii group revealed by host interaction and dna sequence data复杂的进化历史气单胞菌属veronii小组揭示了主机交互和dna序列数据.pdf

Complex Evolutionary History of the Aeromonas veronii Group Revealed by Host Interaction and DNA Sequence Data 1.¤ 1. 1 2,3 1 Adam C. Silver , David Williams , Joshua Faucher , Amy J. Horneman , J. Peter Gogarten , Joerg Graf1* 1 Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, United States of America, 2 Pathology and Laboratory Medical Services, VA Maryland Health Care System, Baltimore, Maryland, United States of America, 3 Department of Medical and Research Technology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America Abstract Aeromonas veronii biovar sobria, Aeromonas veronii biovar veronii, and Aeromonas allosaccharophila are a closely related group of organisms, the Aeromonas veronii Group, that inhabit a wide range of host animals as a symbiont or pathogen. In this study, the ability of various strains to colonize the medicinal leech as a model for beneficial symbiosis and to kill wax worm larvae as a model for virulence was determined. Isolates cultured from the leech out-competed other strains in the leech model, while most strains were virulent in the wax worms. Three housekeeping genes, recA, dnaJ and gyrB, the gene encoding chitinase, chiA, and four loci associated with the type three secretion system, ascV, ascFG, aexT, and aexU were sequenced. The phylogenetic reconstruction failed to produce one consensus tree that was compatible with most of the individual genes. The Approximately Unbiased test and the Genetic Algorithm for Recombination Detection both provided further support for differing evolutionary histories among this group of genes. Two contrasting te