construction of whole genome radiation hybrid panels and map of chromosome 5a of wheat using asymmetric somatic hybridization建设全基因组辐射杂种板和地图使用不对称体细胞杂交小麦染色体5.pdf

Construction of Whole Genome Radiation Hybrid Panels and Map of Chromosome 5A of Wheat Using Asymmetric Somatic Hybridization 1,2. 1. 1¤a 1¤b 1 2 1 2 Chuanen Zhou , Wei Dong , Lu Han , Jiajie Wei , Li Jia , Yang Tan , Daying Zhi , Zeng-Yu Wang , Guangmin Xia1* 1 Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan, Shandong, P.R. China, 2 Forage Improvement Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma, United States of America Abstract To explore the feasibility of constructing a whole genome radiation hybrid (WGRH) map in plant species with large genomes, asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Bupleurum scorzonerifolium Willd. was performed. The protoplasts of wheat were irradiated with ultraviolet light (UV) and gamma-ray and rescued by protoplast fusion using B. scorzonerifolium as the recipient. Assessment of SSR markers showed that the radiation hybrids have the average marker retention frequency of 15.5%. Two RH panels (RHPWI and RHPWII) that contained 92 and 184 radiation hybrids, respectively, were developed and used for mapping of 68 SSR markers in chromosome 5A of wheat. A total of 1557 and 2034 breaks were detected in each panel. The RH map of chromosome 5A based on RHPWII was constructed. The distance of the comprehensive map was 2103 cR and the approximate resolution was estimated to be ,501.6 kb/break. The RH panels evaluated in this study enabled us to order the ESTs in a single deletion bin or in the multiple bins cross the chromosome. These results demonstrated that RH mapp