comparison of c. elegans and c. briggsae genome sequences reveals extensive conservation of chromosome organization and synteny秀丽隐杆线虫和c . briggsae基因组序列的比较揭示了大量保护染色体组织和同线型.pdf

PLoS BIOLOGY Comparison of C. elegans and C. briggsae Genome Sequences Reveals Extensive Conservation of Chromosome Organization and Synteny 1 2 3 1 1 2 LaDeana W. Hillier , Raymond D. Miller , Scott E. Baird , Asif Chinwalla , Lucinda A. Fulton , Daniel C. Koboldt , 4* Robert H. Waterston 1 Genome Sequencing Center, Washington University School of Medicine, Saint Louis, Missouri, United States of America, 2 Department of Genetics, Washington University School of Medicine, Saint Louis, Missouri, United States of America, 3 Department of Biological Sciences, Wright State University, Dayton, Ohio, United States of America, 4 Department of Genome Sciences, University of Washington, Seattle, Washington, United States of America To determine whether the distinctive features of Caenorhabditis elegans chromosomal organization are shared with the C. briggsae genome, we constructed a single nucleotide polymorphism–based genetic map to order and orient the whole genome shotgun assembly along the six C. briggsae chromosomes. Although these species are of the same genus, their most recent common ancestor existed 80–110 million years ago, and thus they are more evolutionarily distant than, for example, human and mouse. We found that, like C. elegans chromosomes, C. briggsae chromosomes exhibit high levels of recombination on the arms along with higher repeat density, a higher fraction of intronic sequence, and a lower fraction of exonic sequence compared with chromosome centers. Despite extensive intrachromosomal rearrangements, 1:1 orthologs tend to remain in the same region of the chromosome, and colinear blocks of orthologs tend to be longer in chromosome centers compared with arms. More strikingly, the two species