whole-genome comparison of two campylobacter jejuni isolates of the same sequence type reveals multiple loci of different ancestral lineage全基因组的比较两个相同空肠弯曲杆菌分离株的序列类型显示多个位点的不同祖先的血统.pdf

Whole-Genome Comparison of Two Campylobacter jejuni Isolates of the Same Sequence Type Reveals Multiple Loci of Different Ancestral Lineage 1 2 3 1 1 Patrick J. Biggs *, Paul Fearnhead , Grant Hotter , Vathsala Mohan , Julie Collins-Emerson , Errol 1 4 5 6 1 Kwan , Thomas E. Besser , Adrian Cookson , Philip E. Carter , Nigel P. French 1 Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand, 2 Department of Mathematics and Statistics, Lancaster University, Lancaster, United Kingdom, 3 Animal Health Section, AgResearch, Hopkirk Research Institute, Palmerston North, New Zealand, 4 Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of America, 5 Rumen Microbiology, Animal Nutrition Health Capability Group, AgResearch Limited, Grasslands Research Centre, Palmerston North, New Zealand, 6 Institute of Environmental Science and Research Ltd., Wellington, New Zealand Abstract Campylobacter jejuni ST-474 is the most important human enteric pathogen in New Zealand, and yet this genotype is rarely found elsewhere in the world. Insight into the evolution of this organism was gained by a whole genome comparison of two ST-474, flaA SVR-14 isolates and other available C. jejuni isolates and genomes. The two isolates were collected from different sources, human (H22082) and retail poultry (P110b), at the same time and from the same geographical location. Solexa sequencing of each isolate resulted in *1.659 Mb (H22082) and *1.656 Mb (P110b) of assembled sequences within 28 (H22082) and 29 (P110b