comparative analyses by sequencing of transcriptomes during skeletal muscle development between pig breeds differing in muscle growth rate and fatness比较分析转录组的测序在骨骼肌发育之间的不同品种的猪在肌肉增长速度和肥胖.pdf

Comparative Analyses by Sequencing of Transcriptomes during Skeletal Muscle Development between Pig Breeds Differing in Muscle Growth Rate and Fatness 1. 1. 1 1 1 1 1 1 Xiao Zhao , Delin Mo , Anning Li , Wen Gong , Shuqi Xiao , Yue Zhang , Limei Qin , Yuna Niu , 1 1 1 1 2 2 1 Yunxue Guo , Xiaohong Liu , Peiqing Cong , Zuyong He , Chong Wang , Jiaqi Li , Yaosheng Chen * 1 State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou, Guangdong, People’s Republic of China, 2 College of Animal Science, South China of Agricultural University, Guangzhou, Guangdong, People’s Republic of China Abstract Understanding the dynamics of muscle transcriptome during development and between breeds differing in muscle growth is necessary to uncover the complex mechanism underlying muscle development. Herein, we present the first transcriptome-wide longissimus dorsi muscle development research concerning Lantang (LT, obese) and Landrace (LR, lean) pig breeds during 10 time-points from 35 days-post-coitus (dpc) to 180 days-post-natum (dpn) using Solexa/Illumina’s Genome Analyzer. The data demonstrated that myogenesis was almost completed before 77 dpc, but the muscle phenotypes were still changed from 77 dpc to 28 dpn. Comparative analysis of the two breeds suggested that myogenesis started earlier but progressed more slowly in LT than in LR, the stages ranging from 49 dpc to 77 dpc are critical for formation of different muscle phenotypes. 595 differentially expressed myogenesis genes were identified,