women have higher protein content of β-oxidation enzymes in skeletal muscle than men女性有更高的蛋白质含量比男性骨骼肌β-oxidation酶.pdf

Women Have Higher Protein Content of b-Oxidation Enzymes in Skeletal Muscle than Men 1 2 3 2 Amy C. Maher , Mahmood Akhtar , Jerry Vockley , Mark A. Tarnopolsky * 1 Department of Medical Science, McMaster University, Hamilton, Ontario, Canada, 2 Department of Pediatrics and Medicine, McMaster University, Hamilton, Ontario, Canada, 3 University of Pittsburgh School of Medicine (Pediatrics) and Graduate School of Public Health (Human Genetics), Pittsburgh, Pennsylvania, United States of America Abstract It is well recognized that compared with men, women have better ultra-endurance capacity, oxidize more fat during endurance exercise, and are more resistant to fat oxidation defects i.e. diet-induced insulin resistance. Several groups have shown that the mRNA and protein transcribed and translated from genes related to transport of fatty acids into the muscle are greater in women than men; however, the mechanism(s) for the observed sex differences in fat oxidation remains to be determined. Muscle biopsies from the vastus lateralis were obtained from moderately active men (N = 12) and women (N = 11) at rest to examine mRNA and protein content of genes involved in lipid oxidation. Our results show that women have significantly higher protein content for tri-functional protein alpha (TFPa), very long chain acyl-CoA dehydrogenase (VLCAD), and medium chain acyl-CoA dehydrogenase (MCAD) (P,0.05). There was no significant sex difference in the expression of short-chain hydroxyacyl-CoA dehydrogenase (SCHAD), or peroxisome proliferator activated receptor alpha (PPARa), or PPARc, genes potentially involved in the transcriptional regulation of lipid metabolism. In conclusion, women have more protein content of the major