antibody-mediated growth inhibition of plasmodium falciparum relationship to age and protection from parasitemia in kenyan children and adults抗体介入增长抑制恶性疟原虫与年龄的关系和保护肯尼亚儿童和成人的寄生虫血症.pdf

Antibody-Mediated Growth Inhibition of Plasmodium falciparum : Relationship to Age and Protection from Parasitemia in Kenyan Children and Adults 1 2 3,4 1 1 Arlene E. Dent , Elke S. Bergmann-Leitner , Danny W. Wilson , Daniel J. Tisch , Rhonda Kimmel , John 5 5 3 2 1 Vulule , Peter Odada Sumba , James G. Beeson , Evelina Angov , Ann M. Moormann , James W. Kazura1* 1 Case Western Reserve University, Cleveland, Ohio, United States of America, 2 Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America, 3 Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia, 4 Department of Medical Biology, University of Melbourne, Melbourne, Victoria, Australia, 5 Kenya Medical Research Institute, Kisumu, Kenya Abstract Background: Antibodies that impair Plasmodium falciparum merozoite invasion and intraerythrocytic development are one of several mechanisms that mediate naturally acquired immunity to malaria. Attempts to correlate anti-malaria antibodies with risk of infection and morbidity have yielded inconsistent results. Growth inhibition assays (GIA) offer a convenient method to quantify functional antibody activity against blood stage malaria. Methods: A treatment-time-to-infection study was conducted over 12-weeks in a malaria holoendemic area of Kenya. Plasma collected from healthy individuals (98 children and 99 adults) before artemether-lumefantrine treatment was tested by GIA in three separate laboratories. Results: Median GIA levels varied with P. falciparum line (D10, 8.8%; 3D7, 34.9%; FVO, 5