牛支原体HB0801株VspX蛋白单克隆抗体的制备与鉴定.pdf
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2017 44 3 868878
China A nim al H usbandry Vete r inary M e d ic ine
doi cnki
j
HB0 80 1 VspX
M y cop lasma bov is M bov is V spX
QuickAntibody M ouse W BALB c
SP V spX
AACH D C IgGb IgG
ELISA
H D
Western blotting D
PG PG D
V spX D V spX
V spX
V spX
S A
Preparation and Identification of the Monoclonal Antibodies against VspX Protein in
My cop lasma bovis strain HB0801
CHEN Xi Zhu Hongmei Z HAO Gang HU Changmin CHEN Ying u GUO Aizhen
y
State K ey L aboratory of A g ricultural M icrobiology College of Veterinary Medicine
H uaz hong A g ricultural Univ ersity Wuhan China
Abstract T o obtain the monoclonal antibody M cA b against V spX protein of M y cop lasm a bov is
M bov is Vsp X gene w as amplified ex pressed and purified T hen BA LB c mice w ere immu
nized subcutaneously three times w ith the purified recombinant V spX rV spX mix ed w ith Quic
kA ntibodyM ouse W adjuvant T hree day s af ter the last injection spleen cells w ere collected
aseptically and f used w ith SP my eloma cells in the presence of poly ethylene glycol By the
clone selection five stable hy bridomas against V spX protein w ere obtained separately named as
A A C H and D A ntibody titers in cell supernatant w ere f rom to
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