高效液相色谱法测定水产品中土霉素_四环素_金霉素.pdf

2007 8 17 8 Chinese Journal ofHealth LaboratoryTechnology, ug2007; Vol17N o 8 1405 高效液相色谱法测定水产品中土霉素四环素金霉素 刘丽, 蔡志斌, 张英 (, 518172) [] : :- 001mol/L , , 0~ 6 m in 04 ml/m in; 6~ 7 min 04~ 08 ml/m in; 7~ 12 min 08 ml/m in : 355 nm , 0010mg/kg, 0008 mg/kg, 0035mg/kg, 004~ 10 mg/kg, 0999, 135%, 85% ~ 120% :, , [] 土霉素; 四环素;金霉素; 高效液相色谱 + [] O6577 2 [] [] 1004- 8685(2007) 08- 1405- 02 D etection of three an tib iotics in aquatic p roducts by HPLC L iu L i, Ca i Zh i-b in, Zhang Y ing ( Longgang Center forD isease Control and Prevention, Shenzhen 518172, China) [ Ab stract] O bjective: To esteblish a smi plemethod for smi ultaneous detection of oxytetracycline, tetracycline, chlortetracycline in aquatic products byHPLCM ethod s: gradient elutionmethod of citric acidw ith 001mol/L acetonitrilewas usedThe flow ratewas 04m l/min in 0~ 6 min, then ascent to 08 ml/m in in the next 2 m in and kept invariable to the endThe detecting wavelength was 355 nmResu ts: The lmi it of detection was 0010 mg/kg for oxytetracycline, 0008 mg/kg for tetracycline, 0035 mg/kg for chlortetracycline, and the linear rangewas 004~ 10 mg/kg( r = 0999)The relative standard deviation was 135% and the recovery was 85% ~ 120% Con c usion: The method is smi ple, sensitive and accurate, and it can be used to de- tect the three antibiotics in aquatic products [ K ey words] Oxytetracycline; Tetracycline; Chlortetracycline; HPLC , M cllvaine ( pH 4): ( Na HPO 2 4 [ 1] GB/T5009116- 2003 , ( 12H O) 276