biofilms of a bacillus subtilis hospital isolate protect staphylococcus aureus from biocide action生物膜的枯草芽孢杆菌医院隔离防止金黄色葡萄球菌杀虫剂行动.pdf

Biofilms of a Bacillus subtilis Hospital Isolate Protect Staphylococcus aureus from Biocide Action 1,2 1,2 1,2,3 ´ 1,2 Arnaud Bridier , Maria del Pilar Sanchez-Vizuete , Dominique Le Coq , Stephane Aymerich , 1,2 4 5 1,2 Thierry Meylheuc , Jean-Yves Maillard , Vincent Thomas , Florence Dubois-Brissonnet , Romain Briandet1,2* 1 INRA, UMR 1319 MICALIS, Jouy-en-Josas, France, 2 AgroParisTech, UMR MICALIS, Jouy-en-Josas, France, 3 CNRS, Jouy-en-Josas, France, 4 Welsh School of Pharmacy, Cardiff University, Cardiff, United Kingdom, 5 STERIS, Fontenay aux Roses, France Abstract The development of a biofilm constitutes a survival strategy by providing bacteria a protective environment safe from stresses such as microbicide action and can thus lead to important health-care problems. In this study, biofilm resistance of a Bacillus subtilis strain (called hereafter NDmedical) recently isolated from endoscope washer-disinfectors to peracetic acid was investigated and its ability to protect the pathogen Staphylococcus aureus in mixed biofilms was evaluated. Biocide action within Bacillus subtilis biofilms was visualised in real time using a non-invasive 4D confocal imaging method. The resistance of single species and mixed biofilms to peracetic acid was quantified using standard plate counting methods and their architecture was explored using confocal imaging and electronic microscopy. The results showed that the NDmedical strain demonstrates the ability to make very large amount of biofilm