comparison of the phenolic content and antioxidant activities of apocynum venetum l. (luo-bu-ma) and two of its alternative species比较对罗布麻生物酚醛含量和抗氧化活性的l(luo-bu-ma)和两个物种的选择.pdf

Int. J. Mol. Sci. 2010, 11, 4452-4464; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Comparison of the Phenolic Content and Antioxidant Activities of Apocynum venetum L. (Luo-Bu-Ma) and Two of Its Alternative Species Taigang Liang, Wenyan Yue and Qingshan Li * School of Pharmaceutical Science, Shanxi Medical University, No 56, Xinjian Nan Road, Taiyuan 030001, Shanxi, China; E-Mails: ltaigang@ (T.L.); yuewenyanhappy@163.com (W.Y.) * Author to whom correspondence should be addressed; E-Mail: qingshanl@; Tel.: +086-351-4690322; Fax: +086-351-4690322. Received: 30 September 2010; in revised form: 17 October 2010 / Accepted: 1 November 2010 / Published: 9 November 2010 Abstract: The leaves of Apocynum venetum L. (AV), a native Chinese plant, have been used as folk medicine in China and Japan. This study evaluated the content of the active antioxidant component and antioxidant activities of AV, and its two alternative species, Poacynum pictum (Schrenk) Baill. (PP) and Poacynum hendersonii (Hook.f.) Woodson (PH). The total phenolic and total flavonoid contents were determined. In addition, the quantitative analysis of two major flavonoid compounds (hyperoside and isoquercitrin) was carried out by HPLC. The antioxidant activities were investigated by the 1,1-diphenyl-2-picrylhydr