the expression of small regulatory rnas in clinical samples reflects the different life styles of staphylococcus aureus in colonization vs. infection小监管rna的表达在临床样本反映出不同的生活风格殖民与金黄色葡萄球菌的感染.pdf

The Expression of Small Regulatory RNAs in Clinical Samples Reflects the Different Life Styles of Staphylococcus aureus in Colonization vs. Infection 1,2,3 2,3 2,3,4 3,4 ` 3,4 1 Juan Song , Claire Lays , Franc¸ois Vandenesch , Yvonne Benito , Michele Bes , Yonglie Chu , ´ 2,3,4 5 2,3 2,3,4 Gerard Lina , Pascale Romby , Thomas Geissmann , Sandrine Boisset * ´ ´ 1 Department of Immunology and Pathogen Biology, Medical school of Xi’an Jiaotong University, Xi’an, Shaanxi province, China, 2 Universite de Lyon, Universite Lyon1, ´ ´ ´ Lyon, France, 3 INSERM U851, Lyon, France, 4 Hospices Civils de Lyon, Centre National de Reference des Staphylocoques, Bron, France, 5 Universite de Strasbourg, CNRS UPR 9002, IBMC, Strasbourg, France Abstract Small RNAs (sRNAs) are involved in the post-transcriptional regulation of metabolic pathways and in responses to stress and virulence. We analyzed the expression levels of five sRNAs of Staphylococcus aureus during human colonization or infection. Total RNA was isolated from nasal carriers, abscesses and cystic fibrosis patients (20 subjects per condition). The expression levels of the sRNAs were measured in the clinical samples and compared with those of the corresponding strains grown in vitro. Five sRNA