β-neurexin is a ligand for the staphylococcus aureus mscramm sdrcβ-neurexin金黄色葡萄球菌发改委mscramm陈叔红的配体.pdf

b-Neurexin Is a Ligand for the Staphylococcus aureus MSCRAMM SdrC 1,2 1 1 2 E. Magda Barbu , Vannakambadi K. Ganesh , Shivasankarappa Gurusiddappa , R. Chris Mackenzie , 3 4 ¨ ¨ 1 Timothy J. Foster , Thomas C. Sudhof , Magnus Hook * 1 Center for Infectious Inflammatory Diseases, Institute of Biosciences and Technology, Texas AM Health Science Center, Houston, Texas, United States of America, 2 Microbiology and Molecular Genetics Department, University of Texas Health Science Center at Houston, Houston, Texas, United States of America, 3 Moyne Institute of Preventive Medicine, Department of Microbiology, Trinity College, Dublin, Ireland, 4 Neuroscience Institute, Department of Molecular and Cellular Physiology, Stanford University, Palo Alto, California, United States of America Abstract Gram-positive bacteria contain a family of surface proteins that are covalently anchored to the cell wall of the organism. These cell-wall anchored (CWA) proteins appear to play key roles in the interactions between pathogenic organisms and the host. A subfamily of the CWA has a common structural organization with multiple domains adopting characteristic IgG-like folds. The identified microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) belong to this subfamily, as does SdrC from S. aureus. However, an interactive host ligand for the putative MSCRAMM SdrC was not previously identified. We have screened a phage display peptide library and identified a peptide sequence found in b- neurexin that binds SdrC. A synthetic peptide corresponding to the identified sequence as well as a recombinant form of the b