uncovering suitable reference proteins for expression studies in human adipose tissue with relevance to obesity发现合适的参考蛋白表达在人类脂肪组织与肥胖的相关性研究.pdf

Uncovering Suitable Reference Proteins for Expression Studies in Human Adipose Tissue with Relevance to Obesity ´ ´ 1,2 ´ 3 ´ 1,2 3 ´ ´ 1,2 Rafael Perez-Perez , Juan A. Lopez , Eva Garcıa-Santos , Emilio Camafeita , Marıa Gomez-Serrano , 4,2 4,2 ´ ´ 4,2 ´ 1,2 Francisco J. Ortega-Delgado , Wifredo Ricart , Jose M. Fernandez-Real , Belen Peral * ´ ´ ´ 1 Instituto de Investigaciones Biomedicas, Alberto Sols, Consejo Superior de Investigaciones Cientıficas (CSIC) Universidad Autonoma de Madrid (UAM), Madrid, Spain, ´ ´ ´ 2 CIBER Fisiopatologıa de la Obesidad y Nutricion (CIBERobn), ISCIII, Madrid, Spain, 3 Unidad de Proteomica, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain, 4 Department of Diabetes, Endocrinology and Nutrition, Hospital Dr. Josep Trueta, Girona, Spain Abstract Background: Protein expression studies based on the two major intra-abdominal human fat depots, the subcutaneous and the omental fat, can shed light into the mechanisms involved in obesity and its co-morbidities. Here we address, for the first time, the identification and validation of reference proteins for data standardization, which are essential for accurate comparison of protein levels in expression stud