the exopolysaccharide matrix modulates the interaction between 3d architecture and virulence of a mixed-species oral biofilm的胞外基质调节之间的交互三维杂交物种口腔生物膜的结构和毒性.pdf

The Exopolysaccharide Matrix Modulates the Interaction between 3D Architecture and Virulence of a Mixed- Species Oral Biofilm 1,2 1 1 3 3 3 Jin Xiao , Marlise I. Klein , Megan L. Falsetta , Bingwen Lu , Claire M. Delahunty , John R. Yates III , Arne Heydorn4, Hyun Koo1,5* 1 Center for Oral Biology, University of Rochester Medical Center, Rochester, New York, United States of America, 2 State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China, 3 Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, United States of America, 4 Department of General Medicine, Glostrup Hospital, Glostrup, Denmark, 5 Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, United States of America Abstract Virulent biofilms are responsible for a range of infections, including oral diseases. All biofilms harbor a microbial-derived extracellular-matrix. The exopolysaccharides (EPS) formed on tooth-pellicle and bacterial surfaces provide binding sites for microorganisms; eventually the accumulated EPS enmeshes microbial cells. The metabolic activity of the bacteria within this matrix leads to acidification of the milieu. We explored the mechanisms through which the Streptococcus mutans-produced EPS-matrix modulates the three-dimensional (3D) architecture and the population shifts during morphogenesis of biofilms on a saliva-coated-apatitic surface using a mixed-bacterial species system. Concomitantly, we examined whether the matrix influences the development of pH-microenvironments within intact-biofilms using a novel 3D in situ pH-mapping technique. Data reveal that the