complex interactions between human myoblasts and the surrounding 3d fibrin-based matrix人类成肌细胞之间复杂的交互和周围的3 d fibrin-based矩阵.pdf

Complex Interactions between Human Myoblasts and the Surrounding 3D Fibrin-Based Matrix ´ 1,2,3,4 1,2,3,4 5 ´ 1,6 ` 1,2,3,4,7 Stephane Chiron , Carole Tomczak , Alain Duperray , Jeanne Laine , Gisele Bonne , 8 8 8 9 1,2,3,4 Alexandra Eder , Arne Hansen , Thomas Eschenhagen , Claude Verdier , Catherine Coirault * 1 Inserm, U974, Paris, France, 2 CNRS, UMR7215, Paris, France, 3 UPMC Univ Paris 06 UM76, IFR14, Paris, France, 4 Institut de Myologie, Paris, France, 5 Inserm, U823, ´ ˆ ` ´ ´ Institut Albert Bonniot, Grenoble, France, 6 UPMC Univ Paris 06, Site Pitie-Salpetriere, Departement de Physiologie, Paris, France, 7 AP-HP, Groupe Hospitalier Pitie- ˆ ` ´ ´ ´ ´ ´ Salpetriere, UF Cardiogenetique et Myogenetique, Service de Biochimie Metabolique, Paris, France, 8 University Medical Center Hamburg-Eppendorf, Hamburg, Germany, ´ 9 CNRS/Universite Grenoble 1, LIPhy UMR 5588, Grenoble, France Abstract Anchorage of muscle cells to the extracellular matrix is crucial for a range of fundamental biological processes including migration, survival and differentiation. Three-dimensional (3D) culture has been proposed to provide a more physiological in vitro model of muscle growth and differentiation than routine 2D cultures. How