biofilm matrix regulation by candida albicans zap1由白色念珠菌生物膜基质调控zap1.pdf

Biofilm Matrix Regulation by Candida albicans Zap1 1,2 3 2 2 4 Clarissa J. Nobile , Jeniel E. Nett , Aaron D. Hernday , Oliver R. Homann , Jean-Sebastien Deneault , 4 3 2 1,5 Andre Nantel , David R. Andes , Alexander D. Johnson , Aaron P. Mitchell * 1 Department of Microbiology, Columbia University, New York, New York, United States of America, 2 Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California, United States of America, 3 Department of Medicine, University of Wisconsin, Madison, Wisconsin, United States of America, 4 Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada, 5 Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America Abstract A biofilm is a surface-associated population of microorganisms embedded in a matrix of extracellular polymeric substances. Biofilms are a major natural growth form of microorganisms and the cause of pervasive device-associated infection. This report focuses on the biofilm matrix of Candida albicans, the major fungal pathogen of humans. We report here that the C. albicans zinc-response transcription factor Zap1 is a negative regulator of a major matrix component, soluble b-1,3 glucan, in both in vitro and in vivo biofilm models. To understand the mechanistic relationship between Zap1 and matrix, we identified Zap1 target genes through expression profiling and full genome chromatin immunoprecipitation. On the basis of these results, we de