conserved and divergent roles of bcr1 and cfem proteins in candida parapsilosis and candida albicans守恒和不同角色bcr1和cfem蛋白质的假丝酵母parapsilosis和白色念珠菌.pdf

Conserved and Divergent Roles of Bcr1 and CFEM Proteins in Candida parapsilosis and Candida albicans 1.¤ 1. 1 2 1 Chen Ding , Genevieve M. Vidanes , Sarah L. Maguire , Alessandro Guida , John M. Synnott , 3 1 David R. Andes , Geraldine Butler * 1 School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Belfield, Dublin, Ireland, 2 School of Medicine and Medical Science, Conway Institute, University College Dublin, Belfield, Dublin, Ireland, 3 Department of Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin, United States of America Abstract Candida parapsilosis is a pathogenic fungus that is major cause of hospital-acquired infection, predominantly due to growth as biofilms on indwelling medical devices. It is related to Candida albicans, which remains the most common cause of candidiasis disease in humans. The transcription factor Bcr1 is an important regulator of biofilm formation in vitro in both C. parapsilosis and C. albicans. We show here that C. parapsilosis Bcr1 is required for in vivo biofilm development in a rat catheter model, like C. albicans. By comparing the transcription profiles of a bcr1 deletion in both species we found that regulation of expression of the CFEM family is conserved. In C. albicans, three of the five CFEM cell wall proteins (Rbt5, Pga7 and Csa1) are associated with both biofilm formation and acquisition of iron from heme, which is an important virulence characteristic. In C. parapsilosis, the CFEM family has undergone an expansion to 7 members. Expression of three genes (CFEM2, CFEM3, and CFEM6) is dependent on Bcr1, and i