widespread occurrence of dosage compensation in candida albicans广泛出现在白色念珠菌剂量补偿.pdf

Widespread Occurrence of Dosage Compensation in Candida albicans 1 2 1 3 4 1 Anatoliy Kravets , Hong Qin , Ausaf Ahmad , Gabor Bethlendy , Qinshan Gao , Elena Rustchenko * 1 Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York, United States of America, 2 Department of Biology, Spelman College, Atlanta, Georgia, United States of America, 3 Roche Diagnostics Corporation, Indianapolis, Indiana, United States of America, 4 Department of Microbiology, Mount Sinai School of Medicine, New York, New York, United States of America Abstract The important human pathogen Candida albicans possesses an unusual form of gene regulation, in which the copy number of an entire specific chromosome or a large portion of a specific chromosome changes in response to a specific adverse environment, thus, insuring survival. In the absence of the adverse environment, the altered portion of the genome can be restored to its normal condition. One major question is how C. albicans copes with gene imbalance arising by transitory aneuploid states. Here, we compared transcriptomes from cells with either two copies or one copy of chromosome 5 (Ch5) in, respectively, a diploid strain 3153A and its representative derivative Sor55. Statistical analyses revealed that at least 40% of transcripts from the monosomic Ch5 are fully compensated to a disomic level, thus, indicating the existence of a genome-wide mechanism maintaining cellular homeostasis. Only approximately 15% of transcripts were diminished twofold in accordance with what would be expected for Ch5 monosomy. Another minor portion of approximately 6% of transcripts, unexpectedly, increased up to t