the candida albicans-specific gene eed1 encodes a key regulator of hyphal extension念珠菌albicans-specific基因eed1编码的关键调节器菌丝的扩展.pdf

The Candida albicans-Specific Gene EED1 Encodes a Key Regulator of Hyphal Extension 1,2,3 4 1 1 1 4 Ronny Martin , Gary P. Moran , Ilse D. Jacobsen , Antje Heyken , Jenny Domey , Derek J. Sullivan , Oliver Kurzai2,3, Bernhard Hube1,3* 1 Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology – Hans Knoell Institute (HKI), Jena, Germany, 2 Group Fungal Septomics, Center for Innovation Competence Septomics, Research at the Leibniz Institute for Natural Product Research and Infection Biology – Hans Knoell Institute (HKI), Jena, Germany, 3 Friedrich Schiller University, Jena, Germany, 4 Microbiology Research Unit, Division of Oral Biosciences, School of Dental Science and Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Dublin, Ireland Abstract The extension of germ tubes into elongated hyphae by Candida albicans is essential for damage of host cells. The C. albicans-specific gene EED1 plays a crucial role in this extension and maintenance of filamentous growth. eed1D cells failed to extend germ tubes into long filaments and switched back to yeast growth after 3 h of incubation during growth on plastic surfaces. Expression of EED1 is regulated by the transcription factor Efg1 and ectopic overexpression of EED1 restored filamentation in efg1D. Transcriptional profiling of eed1D during infection of oral tissue revealed down-regulation of hyphal associated genes including UME6, encoding another key transcriptional factor. Ectopic overexpression of EED1 or UME6 rescued filamentation and damage potential in eed1D. Transcriptional profiling during overexpression of UME6 identified subsets of genes regulated by Eed1