comparative study of hematopoietic differentiation between human embryonic stem cell lines比较研究造血分化之间的人类胚胎干细胞系.pdf

Comparative Study of Hematopoietic Differentiation between Human Embryonic Stem Cell Lines . . Heather Melichar , Ou Li , Jenny Ross, Hilary Haber, Dragana Cado, Hector Nolla, Ellen A. Robey, Astar Winoto* Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, California, United States of America Abstract Directed differentiation of human embryonic stem cells (hESCs) into any desired cell type has been hailed as a therapeutic promise to cure many human diseases. However, substantial roadblocks still exist for in vitro differentiation of hESCs into distinct cell types, including T lymphocytes. Here we examined the hematopoietic differentiation potential of six different hESC lines. We compare their ability to develop into CD34+ + + or CD34 CD45 hematopoietic precursor populations under several differentiation conditions. Comparison of lymphoid potential of hESC derived- and fetal tissue derived- hematopoietic precursors was also made. We found diverse hematopoietic potential between hESC lines depending on the culture or passage conditions. In contrast to fetal-derived hematopoietic precursors, none of the CD34+ precursors differentiated from hESCs were able to develop further into T cells. These data underscore the difficulties in the current strategy of hESC forward differentiation and highlight distinct differences between CD34+ hematopoietic precursors generated in vitro versus in vivo. Citation: Melichar H, Li O, Ross J, Haber H, Cado D, et al. (2011) Comparative Study of Hematopoietic Differentiation between Human Embryoni