tyrosine phosphorylation profiling in fgf-2 stimulated human embryonic stem cells酪氨酸磷酸化剖析在fgf-2刺激了人类胚胎干细胞.pdf

Tyrosine Phosphorylation Profiling in FGF-2 Stimulated Human Embryonic Stem Cells 1,2. 3,4.¤a 1 3,4 1 Vanessa M. Y. Ding , Paul J. Boersema , Leong Yan Foong , Christian Preisinger , Geoffrey Koh , 1 1,2 5 5 3,4¤b Subaashini Natarajan , Dong-Yup Lee , Jos Boekhorst , Berend Snel , Simone Lemeer , Albert J. R. Heck3,4,6*, Andre Choo1,5,7* 1 Stem Cell Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore, 2 Centre for Life Sciences (CeLS), NUS Graduate School for Integrative Sciences and Engineering (NGS), Singapore, Singapore, 3 Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands, 4 Netherlands Proteomics Centre, Utrecht, The Netherlands, 5 Bioinformatics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands, 6 Centre for Biomedical Genetics, Utrecht, The Netherlands, 7 Division of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore, Singapore Abstract The role of fibroblast growth factor-2 (FGF-2) in maintaining undifferentiated human embryonic stem cells (hESC) was investigated using a targeted phosphoproteomics approach to specifically profile tyrosine phosphorylation events following FGF-2 stimulation. A cumulative total number of 735 unique tyrosine phosphorylation sites on 430 proteins were iden