assessment of human multi-potent hematopoietic stemprogenitor cell potential using a single in vitro screening system人类多能造血stemprogenitor细胞潜力的评估使用单一体外筛选系统.pdf

Assessment of Human Multi-Potent Hematopoietic Stem/ Progenitor Cell Potential Using a Single In Vitro Screening System 1,2,3,4 1,2,3,4 5 6 1,2,3,4 Julien Calvo , Aissa BenYoucef , Jan Baijer , Marie-Christine Rouyez , Francoise Pflumio * ´ ¨ ´ ´ ´ 1 CEA, Laboratoire des Cellules Souches Hematopoıetiques et Leucemiques, Institut de Radiobiologie Cellulaire et Moleculaire (IRCM), Fontenay-aux-Roses, France, ´ ´ ´ 2 INSERM, UMR 967, Fontenay-aux-Roses, France, 3 Universite Paris Diderot, UMR 967, Sorbonne Paris Cite, Fontenay-aux-Roses, France, 4 Universite Paris-Sud, UMR 967, Fontenay-aux-Roses, France, 5 CEA, IRCM, Cytometry Service, Fontenay-aux-Roses, France, 6 INSERM, U1016, Paris, France Abstract Hematopoietic stem cells are responsible for the generation of the entire blood system through life. This characteristic relies on their ability to self renew and on their multi-potentiality. Thus quantification of the number of hematopoietic stem cells in a given cell population requires to show both properties in the studied cell populations. Although xenografts models that support human hematopoietic stem cells have been described, such in vivo experimental systems remain restrictive for high throughput screening purposes for example. In this work we developed a conditional tetracycline inducible system controlling the expression