α2,3-sialyltransferase st3gal iii modulates pancreatic cancer cell motility and adhesion in vitro and enhances its metastatic potential in vivoα2,3-sialyltransferase st3gal三世调节胰腺癌细胞的能动性和粘附在体外和体内增强其转移潜力.pdf

a2,3-Sialyltransferase ST3Gal III Modulates Pancreatic Cancer Cell Motility and Adhesion In Vitro and Enhances Its Metastatic Potential In Vivo ´ 1 2 ´ ` 1 1 ` 3 Marta Perez-Garay , Beatriz Arteta , Lluıs Pages , Rafael de Llorens , Carme de Bolos , Fernando Vidal- Vanaclocha2, Rosa Peracaula 1* 1 Biochemistry and Molecular Biology Unit, Department of Biology, University of Girona, Girona, Spain, 2 Department of Cell Biology and Histology, School of Medicine and Dentistry, Basque Country University, Leioa, Spain, 3 Cancer Research Program, IMIM-Hospital del Mar, Barcelona, Spain Abstract Background: Cell surface sialylation is emerging as an important feature of cancer cell metastasis. Sialyltransferase expression has been reported to be altered in tumours and may account for the formation of sialylated tumour antigens. We have focused on the influence of alpha-2,3-sialyltransferase ST3Gal III in key steps of the pancreatic tumorigenic process. Methodology/Principal Findings: ST3Gal III overexpressing pancreatic adenocarcinoma cell lines Capan-1 and MDAPanc-28 were generated. They showed an increase of the tumour associated antigen sialyl-Lewisx. The transfectants’ E-selectin binding capacity was proportional to cell surface sialyl-Lewisx levels. Cellular migration positively correlated with ST3Gal III and sialyl-Lewisx levels. Moreover, intrasplenic injection of the ST3Gal III transfected cells into athymic nude mice showed a decrease in survival and higher metastasis formation when compared to the mock cells. Conclusion: In summary, the