anticancer properties and mechanisms of fucoidan on mouse breast cancer in vitro and in vivo抗癌特性及酶的机制在体外和体内小鼠乳腺癌.pdf

Anticancer Properties and Mechanisms of Fucoidan on Mouse Breast Cancer In Vitro and In Vivo 1 1 1 2 1 1 3 1 Meilan Xue , Yinlin Ge *, Jinyu Zhang , Qing Wang , Lin Hou , Yongchao Liu , Lingling Sun , Quan Li 1 Department of Biochemistry and Molecular Biology, Medical College, Qingdao University, Qingdao, China, 2 Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, China, 3 Department of Pathology, The Affiliated Hospital of Qingdao University, Qingdao, China Abstract Background: Fucoidan is a sulfated polysaccharide derived from brown algae that has been reported to perform multiple biological activities, including antitumor activity. In this study, we examined the influence of crude fucoidan on mouse breast cancer in vitro and in vivo. Materials and Methods: In vitro, fluorescent staining, flow cytometry and Western blot were performed to analyze apoptosis and vascular endothelial growth factor (VEGF) expression of mouse breast cancer 4T1 cells. In vivo, therapy experiments were conducted on Babl/c mice bearing breast cancer. The tumor volume and weight were measured. The number of apoptotic cells and microvascular density (MVD) in tumor tissues were assessed by TUNEL and CD34 immunostaining. Immunohistochemical assays and ELISA assay were used to detect the expression of VEGF in tissues. Results: In vitro studies showed that crude fucoidan significantly decreased the viable number of 4T1 cells, induced apoptosis and down-regulated the expression of VEGF. The expression of Bcl-2 was decreased, and the ratio of Bcl-2 to Bax was significantly decreased. The expression of Survivin and pho