specific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration具体syndecan-1域规范间质肿瘤细胞粘附、能动性和迁移.pdf

Specific Syndecan-1 Domains Regulate Mesenchymal Tumor Cell Adhesion, Motility and Migration 1 . 2. 1 ¨ ´ 1 3 ´ ´ ´ 4 Fang Zong * , Eleni Fthenou , Filip Mundt , Tunde Szatmari , Ilona Kovalszky , Laszlo Szilak , David 5 2 1 1 Brodin , George Tzanakakis , Anders Hjerpe , Katalin Dobra 1 Division of Pathology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden, 2 Division of Morphology, Department of Histology, School of Medicine, University of Crete, Heraklion, Greece, 3 1st Institute of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary, 4 Institute of Biology, Savaria University Center, Western Hungarian University, Szombathely, Hungary, 5 Bioinformatics and Expression Analysis Core Facility, Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden Abstract Background: Syndecans are proteoglycans whose core proteins have a short cytoplasmic domain, a transmembrane domain and a large N-terminal extracellular domain possessing glycosaminoglycan chains. Syndecans are involved in many important cellular processes. Our recent publications have demonstrated that syndecan-1 translocates into the nucleus and hampers tumor cell proliferation. In the present study, we aimed to investigate the role of syndecan-1 in tumor cell adhesion and migration, with special focus on the importance of its distinct protein domains, to better understand the structure-function relationship of syndecan-1 in tumor progression. Methodology/Principal Findings: We utilized two mesenchymal tumor cell lines which were t