comparison of in vitro- and chorioallantoic membrane (cam)-culture systems for cryopreserved medulla-contained human ovarian tissue比较体外,绒毛膜尿囊的膜(cam)文化系统对低温贮藏medulla-contained人类卵巢组织.pdf

Comparison of In Vitro- and Chorioallantoic Membrane (CAM)-Culture Systems for Cryopreserved Medulla- Contained Human Ovarian Tissue 1 1 2,3 1 4 Vladimir Isachenko *, Peter Mallmann , Anna M. Petrunkina , Gohar Rahimi , Frank Nawroth , 5 6 7 7 1 Katharina Hancke , Ricardo Felberbaum , Felicitas Genze , Ilija Damjanoski , Evgenia Isachenko 1 Research Group for Reproductive Medicine and IVF-Laboratory, CAM-Xenotransplantation Group, Department of Obstetrics and Gynecology, Cologne University, Cologne, Germany, 2 Unit of Reproductive Medicine of Clinics, University of Veterinary Medicine, Hannover, Germany, 3 Laboratory of Flow Cytometry, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom, 4 Department for Reproductive Medicine, Center for Endocrinology and Reproductive Medicine in Barkhof, Hamburg, Germany, 5 University Maternal Hospital, Department of Obstetrics and Gynecology, Ulm University, Ulm, Germany, 6 University Maternal Hospital Kempten, Department of Obstetrics and Gynecology, Ulm University, Kempten, Germany, 7 University Hospital for Urology, Ulm University, Ulm, Germany Abstract At present, there are three ways to determine effectively the quality of the cryopreservation procedure using ovarian tissue before the re-implantation treatment: evaluation of follicles after post-thawing xenotransplantation to SCID mouse, in-vitro culture in a large volume of culture medium under constant agitation and culture on embryonic chorio-allantoic membrane within a hen’s eggs. The aim of this study was to compar